Methods


Analytical and spectroscopic methods


Microscopic methods


Microbiological (molecular) methods

  • Denaturing gradient gel electrophoresis (DGGE)
  • Enzyme activity
  • Genetic profiling of microbial community based SSCP (single strand conformation polymorphism)
  • Stable isotope probing (SIP)
  • Quantitative polymerase chain reaction (qPCR)
  • Terminal restriction fragment lenghts polymorphism (TRFLP)
  • 12C/13C PLFA and 12/13C ergosterol analysis


Tomographic methods


Further techniques


Chemical methods

  • standard Density Functional Theory (DFT) based programs (VASP, Turbomole)
  • new efficient DFTB (approx. DF tight-binding) methods


Molecular dynamics

  • DFT, DFTB and FF combined with MD
  • statistical methods for elucidation of MD results


QuantumForce-fields methods

  • standard FF intramolecular parameters will be used
  • interatomic potentials will be represented by the Lennard-Jones potential and atomic charges